Anticorps Monoclonal anti-CD8a

CD8a Monoclonal Antibody for FC, IF

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

IF, FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

53-6.7

N° de cat : CL647-65069

Synonymes

BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2

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Galerie de données de validation

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  IF Staining of mouse splenocytes using CL647-65069

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD8a (CL647-65069, Clone: 53-6.7) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL647-65069

  1X10^6 mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD8a (CL647-65069, Clone:53-6.7) or CoraLite® Plus 647-Conjugated rat IgG2a isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en IF: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65069 cible CD8a dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules spléniques ou membranes de thymocytes
• Nom complet: CD8 antigen, alpha chain
• Numéro d’acquisition GenBank: BC030679
• Symbole du gène: Cd8a
• Identification du gène (NCBI): 12525
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).