Anticorps Monoclonal anti-CD8a

CD8a Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, lambda

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

5H10-1

N° de cat : CL594-65205

Synonymes

BB154331, CD8 antigen, alpha chain, Cd8a, Ly 2, Ly 35, Ly B, Lyt 2



Applications testées

Résultats positifs en cytométriesplénocytes de souris,

Dilution recommandée

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-65205 cible CD8a dans les applications de FC et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Rat / IgG2b, lambda
Clonalité Monoclonal
Type Anticorps
Immunogène Concanavalin A-stimulated BALB/c splenic T cells
Nom complet CD8 antigen, alpha chain
Numéro d’acquisition GenBankBC030679
Symbole du gène Cd8a
Identification du gène (NCBI) 12525
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.09% sodium azide.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocole

Product Specific Protocols
FC protocol for CL594 CD8a antibody CL594-65205Download protocol
Standard Protocols
Click here to view our Standard Protocols