Anticorps Monoclonal anti-CD8a
CD8a Monoclonal Antibody for FC
Hôte / Isotype
Mouse / IgG1, kappa
Réactivité testée
rat
Applications
FC
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
OX-8
N° de cat : CL488-65180
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en cytométrie | splénocytes de rat |
Dilution recommandée
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
CL488-65180 cible CD8a dans les applications de FC et montre une réactivité avec des échantillons rat
Réactivité | rat |
Hôte / Isotype | Mouse / IgG1, kappa |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | s/o |
Nom complet | CD8a molecule |
Numéro d’acquisition GenBank | BC088126 |
Symbole du gène | Cd8a |
Identification du gène (NCBI) | 24930 |
Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Forme | Liquide |
Méthode de purification | Purifié par chromatographie d'affinité par protéine A |
Tampon de stockage | PBS with 0.09% sodium azide. |
Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
Protocole
Product Specific Protocols | |
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FC protocol for CL Plus 488 CD8a antibody CL488-65180 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |