Anticorps Monoclonal anti-CD8a

CD8a Monoclonal Antibody for FC, IF

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

IF, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

OKT8

N° de cat : CL488-65135

Synonymes

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

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Galerie de données de validation

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  IF Staining of human peripheral blood mononuclear cells using CL488-65135

  Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using CoraLite® Plus 488-conjugated Anti-Human CD8a (CL488-65135, Clone: OKT8) at dilution of 1:400.

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  FC experiment of human peripheral blood lymphocytes using CL488-65135

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD3 (APC-65151, Clone: UCHT1) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Human CD8a (CL488-65135, Clone: OKT8). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using CL488-65135

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD3 (APC-65151, Clone: UCHT1) and CoraLite® Plus 488-conjugated Mouse IgG2a isotype control antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en IF: cellules mononucléées de sang périphérique humain,
• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:200-1:800

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65135 cible CD8a dans les applications de IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Thymocytes humains
• Nom complet: CD8a molecule
• Masse moléculaire calculée: 235 aa, 26 kDa
• Numéro d’acquisition GenBank: BC025715
• Symbole du gène: CD8A
• Identification du gène (NCBI): 925
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).