Anticorps Monoclonal anti-CD8a

CD8a Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

rat

Applications

FC

Conjugaison

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

OX-8

N° de cat : CL405-65180

Synonymes

Cd8a, CD8a molecule



Applications testées

Résultats positifs en cytométrieWistar rat splenocytes

Dilution recommandée

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL405-65180 cible CD8a dans les applications de FC et montre une réactivité avec des échantillons rat

Réactivité rat
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet CD8a molecule
Numéro d’acquisition GenBankBC088126
Symbole du gène Cd8a
Identification du gène (NCBI) 24930
Conjugaison CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission maxima wavelengths399 nm / 422 nm
Forme Liquide
Méthode de purification Purifié par chromatographie d'affinité par protéine A
Tampon de stockage PBS with 0.09% sodium azide.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocole

Product Specific Protocols
FC protocol for CL Plus 405 CD8a antibody CL405-65180Download protocol
Standard Protocols
Click here to view our Standard Protocols