Anticorps Monoclonal anti-CD8a

CD8a Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

rat

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

OX-8

N° de cat : APC-65180

Synonymes

Cd8a, CD8a molecule

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Galerie de données de validation

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  FC experiment of rat splenocytes using APC-65180

  1X10^6 rat splenocytes were surface co-stained with PE Anti-Rat CD4 (OX-35) and 0.1 ug APC Anti-Rat CD8a (APC-65180, Clone: OX-8) or 0.1 ug APC Mouse IgG1 Isotype Control (MOPC-21). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de rat

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65180 cible CD8a dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD8a molecule
• Numéro d’acquisition GenBank: BC088126
• Symbole du gène: Cd8a
• Identification du gène (NCBI): 24930
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purifié par chromatographie d'affinité par protéine A
• Tampon de stockage: PBS with 0.1% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T-cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).