Anticorps Monoclonal anti-CD8a

CD8a Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, lambda

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

5H10-1

N° de cat : 65205-1-Ig

Synonymes

BB154331, CD8 antigen, alpha chain, Cd8a, Ly 2, Ly 35, Ly B, Lyt 2

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Galerie de données de validation

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  FC experiment of C57BL/c mouse splenocytes using 65205-1-Ig

  1X10^6 C57BL/c mouse splenocytes were surface co-stained with APC Anti-Mouse CD3ε and 0.25 ug Anti-Mouse CD8a (65205-1-Ig, Clone:5H10-1) and FITC anti-rat IgG2b Antibody at dilution 1:100, or rat IgG2b Isotype Control and FITC anti-rat IgG2b Antibody at dilution 1:100. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65205-1-Ig cible CD8a dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b, lambda
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Concanavalin A-stimulated BALB/c splenic T cells
• Nom complet: CD8 antigen, alpha chain
• Numéro d’acquisition GenBank: BC030679
• Symbole du gène: Cd8a
• Identification du gène (NCBI): 12525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).