Anticorps Polyclonal de lapin anti-CD8a

CD8a Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 29896-1-AP

Synonymes

CD8 antigen, alpha chain, Cd8a, Ly 2, Ly 35, Ly B, Lyt 2

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Galerie de données de validation

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  WB analysis using 29896-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29896-1-AP (CD8a antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse thymus using 29896-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 29896-1-AP (CD8a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse thymus using 29896-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 29896-1-AP (CD8a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 29896-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29896-1-AP (CD8a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 29896-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 29896-1-AP (CD8a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, tissu de thymus de souris
• Résultats positifs en IHC: tissu de thymus de souris, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 5 publications below

Informations sur le produit

29896-1-AP cible CD8a dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD8a Protéine recombinante Ag31942
• Nom complet: CD8 antigen, alpha chain
• Masse moléculaire calculée: 27KD
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: NM_001081110
• Symbole du gène: Cd8a
• Identification du gène (NCBI): 12525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 is composed of two disulfide-linked chains and can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Publications

Species	Application	Title
human	IHC	Front Immunol; Machine learning-based identification of tumor-infiltrating immune cell-associated model with appealing implications in improving prognosis and immunotherapy response in bladder cancer patients; Authors - Hualin Chen
mouse	IHC	Molecules; Immune Enhancement of Clam Peptides on Immunosuppressed Mice Induced by Hydrocortisone; Authors - Meibin Lv
mouse	IHC	J Exp Clin Cancer Res; Regnase-1 downregulation promotes pancreatic cancer through myeloid-derived suppressor cell-mediated evasion of anticancer immunity; Authors - Junya Okabe
mouse	IHC	Bioorg Chem; Discovery of selective and potent USP22 inhibitors via structure-based virtual screening and bioassays exerting anti-tumor activity; Authors - Yue Zhang
mouse	IHC	Biomed Pharmacother; FGF19/FGFR4 signaling contributes to hepatocellular carcinoma survival and immune escape by regulating IGF2BP1-mediated expression of PD-L1; Authors - Chaoqin Guo