Anticorps Monoclonal anti-CD86

CD86 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

IT2.2

N° de cat : CL488-65118

Synonymes

Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

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Galerie de données de validation

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  FC experiment of human PBMCs using CL488-65118

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD86 (CL488-65118, Clone:IT2.2) (red) or unstained. Cells were not fixed. Monocytes were gated.

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  FC experiment of human PBMCs using CL488-65118

  1X10^6 human PBMCs were surface co-stained with APC Anti-Human CD14 and 5 ul CoraLite® Plus 488 Anti-Human CD86 (CL488-65118, Clone:IT2.2) or unstained. Cells were not fixed. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65118 cible CD86 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD86 molecule
• Masse moléculaire calculée: 329 aa, 38 kDa
• Numéro d’acquisition GenBank: BC040261
• Symbole du gène: CD86
• Identification du gène (NCBI): 942
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)