Anticorps Monoclonal anti-CD80

CD80 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

rat

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3H5

N° de cat : CL647-65217

Synonymes

B7 1, Cd80, Cd80 molecule

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Galerie de données de validation

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  FC experiment of rat splenocytes using CL647-65217

  1X10^6 unstimulated or LPS-stimulated rat splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Rat CD45RA (OX-33) and 0.5 ug CoraLite® Plus 647 Anti-Rat CD80 (CL647-65217, Clone: 3H5). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: LPS-stimulated rat splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65217 cible CD80 dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HTLV-1-transformed Lewis-S1 rat T cell line
• Nom complet: Cd80 molecule
• Masse moléculaire calculée: 36 kDa
• Numéro d’acquisition GenBank: XM_017597885
• Symbole du gène: Cd80
• Identification du gène (NCBI): 25408
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)