Anticorps Monoclonal anti-CD80 (B7-1)
CD80 (B7-1) Monoclonal Antibody for FC
Hôte / Isotype
Mouse / IgG1, kappa
Réactivité testée
Humain
Applications
FC
Conjugaison
PE Fluorescent Dye
CloneNo.
2D10.4
N° de cat : PE-65083
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en cytométrie | cellules Daudi, IFN-γ and LPS-stimulated THP-1-derived macrophages |
Dilution recommandée
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
PE-65083 cible CD80 (B7-1) dans les applications de FC et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Hôte / Isotype | Mouse / IgG1, kappa |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | s/o |
Nom complet | CD80 molecule |
Masse moléculaire calculée | 33 kDa |
Numéro d’acquisition GenBank | BC042665 |
Symbole du gène | CD80 |
Identification du gène (NCBI) | 941 |
Conjugaison | PE Fluorescent Dye |
Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
Forme | Liquide |
Méthode de purification | Purification par affinité |
Tampon de stockage | Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2. |
Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
Protocole
Product Specific Protocols | |
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FC protocol for PE CD80 (B7-1) antibody PE-65083 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |