Anticorps Monoclonal anti-CD80 (B7-1)

CD80 (B7-1) Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

2D10.4

N° de cat : PE-65083

Synonymes

Activation B7 1 antigen, B7, B7 1, BB1, CD28LG, CD28LG1, CD80, CD80 (B7-1), CD80 molecule, CTLA 4 counter receptor B7.1, LAB7



Applications testées

Résultats positifs en cytométriecellules Daudi, IFN-γ and LPS-stimulated THP-1-derived macrophages

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65083 cible CD80 (B7-1) dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet CD80 molecule
Masse moléculaire calculée 33 kDa
Numéro d’acquisition GenBankBC042665
Symbole du gène CD80
Identification du gène (NCBI) 941
Conjugaison PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Protocole

Product Specific Protocols
FC protocol for PE CD80 (B7-1) antibody PE-65083Download protocol
Standard Protocols
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