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Anticorps Monoclonal anti-CD8

CD8 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

SK1

N° de cat : CL647-65146

Synonymes

CD8, CD8A, CD8a molecule, Leu2, MAL, p32, SK1

Galerie de données de validation

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD8 (SK1) (CL647-65146)

    FC experiment of human PBMCs using CL647-65146

    1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 405 Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD8 (CL647-65146, Clone:SK1) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD8 (SK1) (CL647-65146)

    FC experiment of human PBMCs using CL647-65146

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD8 (CL647-65146, Clone:SK1) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

Applications testées

Résultats positifs en cytométrieCMSP humaines,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65146 cible CD8 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet CD8a molecule
Masse moléculaire calculée 235 aa, 26 kDa
Numéro d’acquisition GenBankBC025715
Symbole du gène CD8A
Identification du gène (NCBI) 925
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocole

Product Specific Protocols
FC protocol for CL Plus 647 CD8 antibody CL647-65146Download protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sunil (Verified Customer) (07-24-2023)

worked as expected and as per the datasheet, nice to use in a flow panel

  • Applications: Flow Cytometry