Anticorps Monoclonal anti-CD73

CD73 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

Publications

1

CloneNo.

AD2

N° de cat : FITC-65162

Synonymes

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE

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Galerie de données de validation

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  FC experiment of human blood using FITC-65162

  1X10^6 human PBMCs were surface co-stained with Atlantic Blue Anti-Human CD3 and 5 ul FITC Anti-Human CD73 (FITC-65162, Clone:AD2) or unstained. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human blood using FITC-65162

  1X10^6 human PBMCs were surface stained with 5 ul FITC Anti-Human CD73 (FITC-65162, Clone:AD2) or unstained. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: sang humain,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

FITC-65162 cible CD73 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Pre-B leukemia cell line
• Nom complet: 5'-nucleotidase, ecto (CD73)
• Masse moléculaire calculée: 29 kDa, 63 kDa
• Numéro d’acquisition GenBank: BC015940
• Symbole du gène: NT5E
• Identification du gène (NCBI): 4907
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à l'isothiocyanate de fluorescéine (FITC) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Publications

Species	Application	Title
human	FC	Cells; Exosomes from Human Omental Adipose-Derived Mesenchymal Stem Cells Secreted into Ascites Promote Peritoneal Metastasis of Epithelial Ovarian Cancer; Authors - Qingxi Qu