Anticorps Monoclonal anti-CD73

CD73 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

AD2

N° de cat : CL647-65162

Synonymes

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE

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Galerie de données de validation

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  FC experiment of human peripheral blood lymphocytes using CL647-65162

  1X10^6 human peripheral blood lymphocytes were surface stained with PE-Anti-Human CD3 (PE-65148, Clone: SK7) and 5 ul CoraLite® Plus 647-conjugated Anti-Human CD73 (CL647-65162, Clone: AD2). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using CL647-65162

  1X10^6 human peripheral blood lymphocytes were surface stained with PE-Anti-Human CD3 (PE-65148, Clone: SK7) and CoraLite® Plus 647-conjugated mouse IgG1 isotype control antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65162 cible CD73 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Pre-B leukemia cell line
• Nom complet: 5'-nucleotidase, ecto (CD73)
• Masse moléculaire calculée: 29 kDa, 63 kDa
• Numéro d’acquisition GenBank: BC015940
• Symbole du gène: NT5E
• Identification du gène (NCBI): 4907
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).