Anticorps Monoclonal anti-CD73

CD73 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain et plus (1)

Applications

FC

Conjugaison

Non conjugué

Publications

2

CloneNo.

AD2

N° de cat : 65162-1-Ig

Synonymes

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE

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Galerie de données de validation

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  FC experiment of human PBMCs using 65162-1-Ig

  1X10^6 human PBMCs were surface co-stained with CL405 Anti-Human CD3 and 0.5 ug Anti-Human CD73 (65162-1-Ig, Clone:AD2) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or Isotype Control. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using 65162-1-Ig

  1X10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD73 (65162-1-Ig, Clone:AD2) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 2 publications below

Informations sur le produit

65162-1-Ig cible CD73 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Pre-B leukemia cell line
• Nom complet: 5'-nucleotidase, ecto (CD73)
• Masse moléculaire calculée: 29 kDa, 63 kDa
• Numéro d’acquisition GenBank: BC015940
• Symbole du gène: NT5E
• Identification du gène (NCBI): 4907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Publications

Species	Application	Title
human	FC	Exp Ther Med; Adipose-derived mesenchymal stem cell exosomes inhibit transforming growth factor-β1-induced collagen synthesis in oral mucosal fibroblasts.; Authors - Junjie Liu
mouse	FC	Dis Markers; Long Noncoding RNA SBF2-AS1 Promotes Abdominal Aortic Aneurysm Formation through the miRNA-520f-3p/SMARCD1 Axis; Authors - Baihe Han