Anticorps Monoclonal anti-CD72

CD72 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

3F3

N° de cat : PE-65157

Synonymes

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

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Galerie de données de validation

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  FC experiment of human peripheral blood lymphocytes using PE-65157

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD19 (APC-65110, Clone:HIB19) and 20.00 ul/test PE Anti-Human CD72 (PE-65157, Clone:3F3). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using PE-65157

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD19 (APC-65110, Clone:HIB19) and PE mouse IgG2b isotype control antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65157 cible CD72 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes humains normaux provenant d'un ganglion lymphatique
• Nom complet: CD72 molecule
• Numéro d’acquisition GenBank: BC011734
• Symbole du gène: CD72
• Identification du gène (NCBI): 971
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à la R-phycoérithrine (PE) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS avec azoture de sodium à 0,1 %.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).