Anticorps Monoclonal anti-CD69

CD69 Monoclonal Antibody for FC

Hôte / Isotype

Armenian Hamster / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

H1.2F3

N° de cat : CL555-65105

Synonymes

5830438K24Rik, AI452015, AIM, Cd69, CD69 antigen, VEA

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL555-65105

  1X10^6 unstimulated or anti-CD3/CD28-stimulated mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3) or 0.5 ug Isotype Control. Cells were not fixed.

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  FC experiment of mouse splenocytes using CL555-65105

  1X10^6 anti-CD3/CD28-stimulated mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3) (red), or 0.5 ug Isotype Control (blue). The black dashed line indicates unstimulated mouse splenocytes surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28-stimulated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-65105 cible CD69 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Armenian Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée de lymphocytes T épidermiques dendritiques de souris Y245
• Nom complet: CD69 antigen
• Numéro d’acquisition GenBank: BC106997
• Symbole du gène: Cd69
• Identification du gène (NCBI): 12515
• Conjugaison: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).