Anticorps Monoclonal anti-CD64

CD64 Monoclonal Antibody for FC, ELISA

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC, ELISA

Conjugaison

Non conjugué

CloneNo.

10.1

N° de cat : 65253-1-Ig

Synonymes

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using 65253-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD64 (65253-1-Ig, Clone:10.1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65253-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD64 (65253-1-Ig, Clone:10.1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

---

Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65253-1-Ig cible CD64 dans les applications de FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
• Nom complet: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Masse moléculaire calculée: 374 aa, 43 kDa
• Numéro d’acquisition GenBank: BC032634
• Symbole du gène: FCGR1A
• Identification du gène (NCBI): 2209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.