Anticorps Monoclonal anti-CD63

CD63 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3D4D1

Avis (1)

N° de cat : CL594-67605

Synonymes

CD63, CD63 antigen, CD63 molecule, Granulophysin, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30

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Galerie de données de validation

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  IF Staining of human tonsillitis using CL594-67605

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated CD63 antibody (CL594-67605, Clone: 3D4D1 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-67605

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated CD63 antibody (CL594-67605, Clone: 3D4D1 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-67605

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated CD63 antibody (CL594-67605, Clone: 3D4D1 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF: tissu d'amygdalite humain,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67605 cible CD63 dans les applications de IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD63 Protéine recombinante Ag19690
• Nom complet: CD63 molecule
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC002349
• Symbole du gène: CD63
• Identification du gène (NCBI): 967
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Amr (Verified Customer) (10-07-2021)

Anti CD63 CL488 and CL594 were validated in Blood plasma exosomes and in bone marrow. The antibodies seem to recogize their specific epitopes very well both extra and intracellularly. Strongly recommended.!

• Applications: Flow Cytometry
• Primary Antibody Dilution: 0.5:10 CL495 and 1:10 CL488
• Cell Tissue Type: Blood plasma/ Bone marrow

CL594 CD63 Antibody Flow Cytometry validation (0.5:10 CL495 and 1:10 CL488 dilution) in Blood plasma/ Bone marrow (Cat no:CL594-67605)