Anticorps Monoclonal anti-CD62L (L-Selectin)

CD62L (L-Selectin) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

FITC Fluorescent Dye

CloneNo.

MEL-14

N° de cat : FITC-65123

Synonymes

CD62L (L-Selectin), Sell

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Galerie de données de validation

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  FC experiment of mouse splenocytes using FITC-65123

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.25 ug FITC-Anti-Mouse CD62L (L-Selectin) (FITC-65123, Clone: MEL-14). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-65123

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.25 ug FITC-Rat IgG2a isotype control antibody. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-65123

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.06 ug FITC Anti-Mouse CD62L (L-Selectin) (FITC-65123, Clone:MEL-14) (red) or 0.06 ug FITC-rat IgG2a isotype control (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65123 cible CD62L (L-Selectin) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphome B de souris C3H/eb 38C-13
• Nom complet: selectin, lymphocyte
• Numéro d’acquisition GenBank: BC052681
• Symbole du gène: Sell
• Identification du gène (NCBI): 20343
• Conjugaison: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 498 nm / 526 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).