Anticorps Monoclonal anti-CD54 (ICAM-1)

CD54 (ICAM-1) Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

15.2

N° de cat : CL555-65075

Synonymes

BB2, CD54, ICAM 1, ICAM1, P3.58

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Galerie de données de validation

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  FC experiment of human PBMCs using CL555-65075

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD54 (ICAM-1) (CL555-65075, Clone:15.2) (red) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL555-65075

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD54 (ICAM-1) (CL555-65075, Clone:15.2) or unstained. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-65075 cible CD54 (ICAM-1) dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules synoviales rhumatoïdes et monocytes humains
• Nom complet: intercellular adhesion molecule 1
• Masse moléculaire calculée: 90 kDa
• Numéro d’acquisition GenBank: BC015969
• Symbole du gène: ICAM1
• Identification du gène (NCBI): 3383
• Conjugaison: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).