Anticorps Monoclonal anti-CD49b

CD49b Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgM, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

DX5

N° de cat : CL488-65065

Synonymes

CD49B, DX5, integrin alpha 2, Itga2

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL488-65065

  1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse NK1.1 (CD161) (PK136) (APC-65138, Clone: PK136) and CoraLite® Plus 488-conjugated Rat IgM isotype control antibody (left) or 5.00 ul CoraLite® Plus 488-conjugated Anti-Mouse CD49b (CL488-65065, Clone: DX5) (right). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65065 cible CD49b dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgM, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules NK isolées de souris C57BL/6
• Nom complet: integrin alpha 2
• Numéro d’acquisition GenBank: BC065139
• Symbole du gène: Itga2
• Identification du gène (NCBI): 16398
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and CLG gene expression, force generation and organization of newly synthesized extracellular matrix.