Anticorps Monoclonal anti-CD45RA

CD45RA Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

rat

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

OX-33

N° de cat : CL647-65234

Synonymes

CD45, CD45RA, L CA, Lca, Ptprc, RT7, T200

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Galerie de données de validation

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  FC experiment of rat splenocytes using CL647-65234

  1X10^6 rat splenocytes cells were surface co-stained with FITC Anti-Rat CD4 (OX-35) and 0.5 ug CoraLite® Plus 647 Anti-Rat CD45RA (CL647-65234, Clone: OX-33) or 0.5 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: rat splenocytes cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65234 cible CD45RA dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Leukocyte common antigen purified from rat splenocytes
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Masse moléculaire calculée: 143 kDa
• Numéro d’acquisition GenBank: XM_006249910
• Symbole du gène: Ptprc
• Identification du gène (NCBI): 24699
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).