Anticorps Monoclonal anti-CD45RA

CD45RA Monoclonal Antibody for FC, IF

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

IF, FC

Conjugaison

APC Fluorescent Dye

CloneNo.

HI100

N° de cat : APC-65108

Synonymes

B220, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Galerie de données de validation

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  IF Staining of human peripheral blood mononuclear cells using APC-65108

  Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using APC Anti-Human CD45RA (APC-65108, Clone: HI100) and CoraLite®488-conjugated Anti-Human CD45RO (CL488-65150, Clone: UCHL1) at dilution of 1:300.

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  FC experiment of human peripheral blood lymphocytes using APC-65108

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.125 ug APC-Anti-Human CD45RA (APC-65108, clone HI100) (red) or 0.125 ug APC-mouse IgG2b isotype control (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en IF: cellules mononucléées de sang périphérique humain,
• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:150-1:600

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65108 cible CD45RA dans les applications de IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: PTPRC
• Identification du gène (NCBI): 5788
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).