Anticorps Monoclonal anti-CD45RA

CD45RA Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

F8-11-13

N° de cat : 65226-1-Ig

Synonymes

B220, CD45, CD45R, CD45RA, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using 65226-1-Ig

  1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3, FITC Anti-human CD45RO, and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65226-1-Ig

  1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3 and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

---

Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65226-1-Ig cible CD45RA dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Human T lymphocytes
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Masse moléculaire calculée: 1304 aa, 147 kDa
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: PTPRC
• Identification du gène (NCBI): 5788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).