Anticorps Monoclonal anti-CD45R (B220)

CD45R (B220) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

Humain, souris

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

RA3-6B2

N° de cat : FITC-65139

Synonymes

B220, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Galerie de données de validation

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  FC experiment of mouse splenocytes using FITC-65139

  1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD45R (B220) (FITC-65139, Clone: RA3-6B2) (red), or 0.5 ug Control Antibody. Cells were not fixed.

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  FC experiment of mouse splenocytes using FITC-65139

  1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and 0.5 ug FITC-Rat IgG2a isotype control (left) or 0.5 ug FITC Anti-Mouse CD45R (B220) (FITC-65139, Clone: RA3-6B2) (right). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65139 cible CD45R (B220) dans les applications de FC et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules tumorales pré-B induites par le virus de la leucémie murine d'Abelson
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC028512
• Symbole du gène: Ptprc
• Identification du gène (NCBI): 19264
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.