Anticorps Monoclonal anti-CD45

CD45 Monoclonal Antibody for FC, IF

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF, FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

HI30

N° de cat : CL647-65109

Synonymes

B220, CD45, CD45R, GP180, HI30, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Galerie de données de validation

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  IF Staining of Jurkat using CL647-65109

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 647 CD45 antibody (CL647-65109, Clone: HI30 ) at dilution of 1:200.

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  FC experiment of human PBMCs using CL647-65109

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD45 (CL647-65109, Clone: HI30), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en IF: cellules Jurkat,
• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65109 cible CD45 dans les applications de IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Human peripheral blood leucocytes
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: PTPRC
• Identification du gène (NCBI): 5788
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).