Anticorps Recombinant de lapin anti-CD45

CD45 Recombinant Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

6O19

Avis (1)

N° de cat : CL555-80297

Synonymes

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Galerie de données de validation

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  IF Staining of mouse spleen using CL555-80297

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite®555 CD45 antibody (CL555-80297, Clone: 6O19 ) at dilution of 1:200.

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  IF Staining of mouse spleen using CL555-80297

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite®555 CD45 antibody (CL555-80297, Clone: 6O19 ) at dilution of 1:200.

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  FC experiment of Raji using CL555-80297

  1X10^6 Raji cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human CD45 (CL555-80297, Clone:6O19) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu splénique de souris,
• Résultats positifs en cytométrie: cellules Raji

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-80297 cible CD45 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD45 Protéine recombinante Ag13712
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Masse moléculaire calculée: 1304 aa, 147 kDa
• Poids moléculaire observé: 220-240 kDa
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: PTPRC
• Identification du gène (NCBI): 5788
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sonja (Verified Customer) (01-27-2023)

The antibody is perfect. No background and very strong specific signal!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: FFPE Tonsil

CL555 CD45 Antibody Immunofluorescence validation (1:50 dilution) in FFPE Tonsil (Cat no:CL555-80297)