Anticorps Monoclonal anti-CD45

CD45 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

2D1

N° de cat : 65082-1-Ig

Synonymes

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY 5, LY5, PTPRC, RPTPC, T200

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Galerie de données de validation

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  FC experiment of human peripheral blood lymphocytes using 65082-1-Ig

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD45 (65082-1-Ig, Clone: 2D1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65082-1-Ig cible CD45 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CMSP humaines
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: PTPRC
• Identification du gène (NCBI): 5788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).