Anticorps Monoclonal anti-CD45.2

CD45.2 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

104

N° de cat : CL555-65072

Synonymes

B220, Cd45, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200

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Galerie de données de validation

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  FC experiment of C57BL/6 mouse splenocytes using CL555-65072

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD45.2 (CL555-65072, Clone:104), or 0.5 ug CoraLite® Plus 555 Mouse IgG2a Isotype Control (C1.18.4) (CL555-65208, Clone: C1.18.4). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: C57BL/6 mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-65072 cible CD45.2 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Thymocytes et splénocytes de souris B10.S
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC028512
• Symbole du gène: Ptprc
• Identification du gène (NCBI): 19264
• Conjugaison: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).