Anticorps Monoclonal anti-CD44

CD44 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

IF, FC

Conjugaison

FITC Plus Fluorescent Dye

Publications

3

CloneNo.

F10-44-2

N° de cat : FITC-65063

Synonymes

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using FITC-65063

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD44 (FITC-65063, Clone: F10-44-2), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

---

Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below
• FC: See 2 publications below

Informations sur le produit

FITC-65063 cible CD44 dans les applications de IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes T purifiées de ganglions lymphatiques humains
• Nom complet: CD44 molecule (Indian blood group)
• Masse moléculaire calculée: 742 aa, 82 kDa
• Numéro d’acquisition GenBank: BC004372
• Symbole du gène: CD44
• Identification du gène (NCBI): 960
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Publications

Species	Application	Title
human	FC	ACS Nano; Inhibition Effect of Ti3C2Tx MXene on Ice Crystals Combined with Laser-Mediated Heating Facilitates High-Performance Cryopreservation.; Authors - Yuan Cao
human	IF	Free Radic Biol Med; Retinal cell-targeted liposomal ginsenoside Rg3 attenuates retinal ischemia-reperfusion injury via alleviating oxidative stress and promoting microglia/macrophage M2 polarization; Authors - Yanmei Huang
human	FC	Open Life Sci; Experimental study on the optimization of ANM33 release in foam cells; Authors - Chen Yuan