Anticorps Monoclonal anti-CD44

CD44 Monoclonal Antibody for FC, IF

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

Humain, souris

Applications

IF, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

IM7

N° de cat : CL488-65117

Synonymes

AU023126, AW121933, AW146109, Cd44, CD44 antigen, HERMES, Ly 24, Pgp 1

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Galerie de données de validation

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  IF Staining of mouse splenocytes using CL488-65117

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488 CD44 antibody (CL488-65117, Clone: IM7 ) at dilution of 1:100.

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  FC experiment of mouse splenocytes using CL488-65117

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and CoraLite® Plus 488 conjugated Rat IgG2b (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD44 (CL488-65117, Clone: IM7) (right). Cells were not fixed.

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Applications testées

• Résultats positifs en IF: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65117 cible CD44 dans les applications de IF, FC et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules M1 de leucémie myéloïde induite par la dexaméthasone
• Nom complet: CD44 antigen
• Numéro d’acquisition GenBank: BC051388
• Symbole du gène: Cd44
• Identification du gène (NCBI): 12505
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.