Anticorps Monoclonal anti-CD40

CD40 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

FITC Fluorescent Dye

Publications

2

CloneNo.

1C10

N° de cat : FITC-65062

Synonymes

AI326936, Bp50, Cd40, CD40 antigen, GP39, HIGM1, IGM, IMD3, p50, T BAM, Tnfrsf5

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Galerie de données de validation

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  FC experiment of BALB/c mouse splenocytes using FITC-65062

  1X10^6 BALB/c mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.20 ug FITC-Anti-Mouse CD40 (FITC-65062, Clone: 1C10). Cells were not fixed.

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  FC experiment of BALB/c mouse splenocytes using FITC-65062

  1X10^6 BALB/c mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.20 ug FITC-Rat IgG2a isotype control antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris BALB/c,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 2 publications below

Informations sur le produit

FITC-65062 cible CD40 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: sCD40
• Nom complet: CD40 antigen
• Numéro d’acquisition GenBank: BC029254
• Symbole du gène: Cd40
• Identification du gène (NCBI): 21939
• Conjugaison: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 494 nm / 520 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 %.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

Publications

Species	Application	Title
mouse	FC	Int Immunopharmacol; Mesenchymal stem cell-derived extracellular vesicles carrying miR-99b-3p restrain microglial activation and neuropathic pain by stimulating autophagy; Authors - Xu Gao
mouse	FC	Int Immunopharmacol; Huc-MSCs-derived exosomes attenuate neuropathic pain by inhibiting activation of the TLR2/MyD88/NF-κB signaling pathway in the spinal microglia by targeting Rsad2; Authors - Xu Gao