Anticorps Monoclonal anti-CD40

CD40 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / Mouse IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

G28.5

N° de cat : CL488-65103

Synonymes

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

---

Galerie de données de validation

• 

  FC experiment of human peripheral blood lymphocytes using CL488-65103

  1X10^6 human peripheral blood lymphocytes were surface stained with 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD40 (CL488-65103, Clone: G28.5) (red) or isotype control antibody (blue). Cells were not fixed.

• 

  FC experiment of Raji using CL488-65103

  1X10^6 Raji cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD40 (CL488-65103, Clone:G28.5) (red), or 5 ul Control Antibody. Cells were not fixed .

---

Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain, cellules Raji

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65103 cible CD40 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / Mouse IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD40 molecule, TNF receptor superfamily member 5
• Masse moléculaire calculée: 277 aa, 31 kDa
• Numéro d’acquisition GenBank: BC012419
• Symbole du gène: CD40
• Identification du gène (NCBI): 958
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à une température comprise entre 2 °C et 8 °C. Éviter toute exposition à la lumière.

Informations générales

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).