Anticorps Monoclonal anti-CD40

CD40 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

G28.5

N° de cat : APC-65103

Synonymes

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

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Galerie de données de validation

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  FC experiment of human peripheral blood lymphocytes using APC-65103

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.20 ug APC-anti-human CD40 (APC-65103, clone G28.5) (red) or 0.20 ug APC-mouse IgG1 isotype control (blue). Cells were not fixed.

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  FC experiment of Raji using APC-65103

  1X10^6 Raji cells were surface stained with 5 ul APC Anti-Human CD40 (APC-65103, Clone: G28.5) (red) or Control Antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain, cellules Raji

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65103 cible CD40 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD40 molecule, TNF receptor superfamily member 5
• Masse moléculaire calculée: 277 aa, 31 kDa
• Numéro d’acquisition GenBank: BC012419
• Symbole du gène: CD40
• Identification du gène (NCBI): 958
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).