Anticorps Monoclonal anti-CD4

CD4 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

PE Fluorescent Dye

Publications

2

CloneNo.

RM4-5

N° de cat : PE-65141

Synonymes

Cd4, CD4 antigen, L3T4, Ly 4

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Galerie de données de validation

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  FC experiment of C57BL/6 mouse splenocytes using PE-65141

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 (17A2) and 0.25 ug PE Anti-Mouse CD4 (PE-65141, Clone:RM4-5) or 0.25 ug PE Rat IgG2a Isotype Control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 2 publications below

Informations sur le produit

PE-65141 cible CD4 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: thymocytes de souris BALB/c
• Nom complet: CD4 antigen
• Numéro d’acquisition GenBank: BC039137
• Symbole du gène: Cd4
• Identification du gène (NCBI): 12504
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publications

Species	Application	Title
mouse	FC	J Thromb Haemost; Enhanced metabolic activation of and platelet response to clopidogrel in T-cell deficient mice through induction of Cyp2c and Cyp3a and inhibition of Ces1; Authors - Li-Ping Jiang
mouse	FC	Cell Death Discov; TRAF6 triggers Mycobacterium-infected host autophagy through Rab7 ubiquitination; Authors - Qinmei Ma