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Anticorps Monoclonal anti-CD4

CD4 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

SK3

N° de cat : CL647-65147

Synonymes

CD4, CD4 molecule, CD4mut, SK3

Galerie de données de validation

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD4 (SK3) (CL647-65147)

    FC experiment of human PBMCs using CL647-65147

    1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 405 Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD4 (CL647-65147, Clone:SK3) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD4 (SK3) (CL647-65147)

    FC experiment of human PBMCs using CL647-65147

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD4 (CL647-65147, Clone:SK3) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

Applications testées

Résultats positifs en cytométrieCMSP humaines,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65147 cible CD4 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet CD4 molecule
Masse moléculaire calculée 55 kDa
Numéro d’acquisition GenBankBC025782
Symbole du gène CD4
Identification du gène (NCBI) 920
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocole

Product Specific Protocols
FC protocol for CL Plus 647 CD4 antibody CL647-65147Download protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sunil (Verified Customer) (07-28-2023)

Used on cytotrols absolutely fine as a control conjugate for our own custom conjugates

  • Applications: Flow Cytometry
FH

Sareena (Verified Customer) (07-25-2023)

Works well as duplex stain

  • Applications: Flow Cytometry