Anticorps Monoclonal anti-CD4

CD4 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

RM4-5

N° de cat : APC-65141

Synonymes

Cd4, CD4 antigen, L3T4, Ly 4

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Galerie de données de validation

• 

  FC experiment of mouse splenocytes using APC-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.13 ug APC Anti-Mouse CD4 (APC-65141, Clone: RM4-5). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using APC-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.13 ug APC-Rat IgG2a isotype control antibody. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using APC-65141

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20 ug APC-anti-mouse CD4 (APC-65141, clone RM4-5) (red) or 0.20 ug APC-rat IgG2a isotype control (blue). Cells were not fixed. .

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65141 cible CD4 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: thymocytes de souris BALB/c
• Nom complet: CD4 antigen
• Numéro d’acquisition GenBank: BC039137
• Symbole du gène: Cd4
• Identification du gène (NCBI): 12504
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).