Anticorps Monoclonal anti-CD38

CD38 Monoclonal Antibody for FC, IF

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

IF, FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

90

N° de cat : CL647-65059

Synonymes

Cd38, CD38 antigen, Cd38 rs1

---

Galerie de données de validation

• 

  IF Staining of mouse splenocytes using CL647-65059

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD38 (CL647-65059, Clone: 90) at dilution of 1:400.

• 

  FC experiment of mouse splenocytes using CL647-65059

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3ε (FITC-65060, Clone: 145-2C11) and CoraLite® Plus 647-conjugated Rat IgG2a isotype control (left) or 5.00 ul CoraLite® Plus 647-conjugated Anti-Mouse CD38 (CL647-65059, Clone: 90) (right). Cells were not fixed.

---

Applications testées

• Résultats positifs en IF: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:200-1:800

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65059 cible CD38 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules pré-B médullaires de souris
• Nom complet: CD38 antigen
• Numéro d’acquisition GenBank: BC046312
• Symbole du gène: Cd38
• Identification du gène (NCBI): 12494
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).