Anticorps Monoclonal anti-CD38

CD38 Monoclonal Antibody for FC, IF

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

HB7

N° de cat : CL488-65107

Synonymes

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

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Galerie de données de validation

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  IF Staining of human peripheral blood mononuclear cells using CL488-65107

  Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using CoraLite® Plus 488-conjugated Anti-Human CD38 (CL488-65107, Clone:HB7) at dilution of 1:400.

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  FC experiment of human peripheral blood lymphocytes using CL488-65107

  100 ul human peripheral blood were surface stained 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD38 (CL488-65107, Clone:HB7) (red) or isotype control antibody (blue). Lymphocytes were gated for analysis. Cells were not fixed.

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Applications testées

• Résultats positifs en IF: cellules mononucléées de sang périphérique humain,
• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:200-1:800

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65107 cible CD38 dans les applications de IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée cellulaire B BJAB humaine
• Nom complet: CD38 molecule
• Masse moléculaire calculée: 300 aa, 34 kDa
• Numéro d’acquisition GenBank: BC007964
• Symbole du gène: CD38
• Identification du gène (NCBI): 952
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).