Anticorps Monoclonal anti-CD35

CD35 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Atlantic Blue™ Fluorescent Dye

CloneNo.

E11

N° de cat : AB-65254

Synonymes

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN



Applications testées

Résultats positifs en cytométrieCMSP humaines,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

AB-65254 cible CD35 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Human CD35 from Acute monocytic leukemia cells and normal blood monocytes
Nom complet complement component (3b/4b) receptor 1 (Knops blood group)
Masse moléculaire calculée 224 kDa
Numéro d’acquisition GenBankNM_000573
Symbole du gène CR1
Identification du gène (NCBI) 1378
Conjugaison Atlantic Blue™ Fluorescent Dye
Excitation/Emission maxima wavelengths404 nm / 458 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide and 0.5% BSA.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

Protocole

Product Specific Protocols
FC protocol for Atlantic Blue™ CD35 antibody AB-65254Download protocol
Standard Protocols
Click here to view our Standard Protocols