Anticorps Monoclonal anti-CD34

CD34 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

4F11H3

N° de cat : 60180-2-Ig

Synonymes

CD34, CD34 molecule

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Galerie de données de validation

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  WB analysis of human placenta using 60180-2-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human uterus using 60180-2-Ig

  human uterus tissue were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 60180-2-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ovary cancer using 60180-2-Ig

  human ovary cancer cells were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu de cancer de l'ovaire humain, tissu testiculaire humain, tissu utérin humain
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

60180-2-Ig cible CD34 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD34 Protéine recombinante Ag5996
• Nom complet: CD34 molecule
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC039146
• Symbole du gène: CD34
• Identification du gène (NCBI): 947
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).

Publications

Species	Application	Title
human	IHC	Front Oncol; Prognostic Impact of the Angiogenic Gene POSTN and Its Related Genes on Lung Adenocarcinoma.; Authors - Dongfeng Sun
		BMC Cancer; Novel fusion protein PK5-RL-Gal-3C inhibits hepatocellular carcinoma via anti-angiogenesis and cytotoxicity; Authors - Xiaoge Gao