Anticorps Monoclonal anti-CD31

CD31 Monoclonal Antibody for IF,FC

Hôte / Isotype

Rat / IgG2a

Réactivité testée

souris

Applications

IF,FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

390

N° de cat : CL647-65058

Synonymes

C85791, Cd31, Pecam, PECAM 1, Pecam1

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Galerie de données de validation

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  IF Staining of bEnd.3 using CL647-65058

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite® Plus 647 CD31 antibody (CL647-65058, Clone: 390) at dilution of 1:100.

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  FC experiment of mouse splenocytes using CL647-65058

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 5.00 ul/test CoraLite® Plus 647-conjugated Anti-Mouse CD31 (CL647-65058, Clone: 390) (red) or isotype control antibody (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en IF: cellules bEnd.3,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65058 cible CD31 dans les applications de IF,FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée de cellules progénitrices hématopoïétiques 32D de souris C3H/HeJ
• Nom complet: platelet/endothelial cell adhesion molecule 1
• Numéro d’acquisition GenBank: BC008519
• Symbole du gène: Pecam1
• Identification du gène (NCBI): 18613
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à une température comprise entre 2 °C et 8 °C. Éviter toute exposition à la lumière.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).