Anticorps Monoclonal anti-CD31
CD31 Monoclonal Antibody for FC, IF
Hôte / Isotype
Rat / IgG2a, kappa
Réactivité testée
souris
Applications
IF, FC
Conjugaison
CoraLite®594 Fluorescent Dye
CloneNo.
390
N° de cat : CL594-65058
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en IF | cellules bEnd.3, |
Résultats positifs en cytométrie | splénocytes de souris, |
Dilution recommandée
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
CL594-65058 cible CD31 dans les applications de IF, FC et montre une réactivité avec des échantillons souris
Réactivité | souris |
Hôte / Isotype | Rat / IgG2a, kappa |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | Lignée de cellules progénitrices hématopoïétiques 32D de souris C3H/HeJ |
Nom complet | platelet/endothelial cell adhesion molecule 1 |
Numéro d’acquisition GenBank | BC008519 |
Symbole du gène | Pecam1 |
Identification du gène (NCBI) | 18613 |
Conjugaison | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Forme | Liquide |
Méthode de purification | Purification par affinité |
Tampon de stockage | PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3. |
Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
Protocole
Product Specific Protocols | |
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IF protocol for CL594 CD31 antibody CL594-65058 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |