Anticorps Monoclonal anti-CD31

CD31 Monoclonal Antibody for FC, IF

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

IF, FC

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

390

N° de cat : CL594-65058

Synonymes

C85791, Cd31, Pecam, PECAM 1, Pecam1



Applications testées

Résultats positifs en IFcellules bEnd.3,
Résultats positifs en cytométriesplénocytes de souris,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-65058 cible CD31 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Rat / IgG2a, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Lignée de cellules progénitrices hématopoïétiques 32D de souris C3H/HeJ
Nom complet platelet/endothelial cell adhesion molecule 1
Numéro d’acquisition GenBankBC008519
Symbole du gène Pecam1
Identification du gène (NCBI) 18613
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocole

Product Specific Protocols
IF protocol for CL594 CD31 antibody CL594-65058Download protocol
Standard Protocols
Click here to view our Standard Protocols