Anticorps Polyclonal de lapin anti-CD31

• Phare
• Validé par KD/KO

CD31 Polyclonal Antibody for IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

Publications

1

N° de cat : CL594-11265

Synonymes

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

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Galerie de données de validation

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  IF Staining of human breast cancer using CL594-11265

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200, CoraLite®488 Cytokeratin 7 antibody (CL488-15539, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, magenta).

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  IF Staining of human breast cancer using CL594-11265

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200, CoraLite®488 Cytokeratin 7 antibody (CL488-15539, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, magenta).

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  IF Staining of HUVEC using CL594-11265

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-11265

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-11265

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF: tissu de cancer du sein humain, cellules HUVEC, tissu d'amygdalite humain

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-11265 cible CD31 dans les applications de IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD31 Protéine recombinante Ag1787
• Nom complet: platelet/endothelial cell adhesion molecule
• Masse moléculaire calculée: 83 kDa
• Numéro d’acquisition GenBank: BC022512
• Symbole du gène: PECAM1
• Identification du gène (NCBI): 5175
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
human	IF	Stem Cell Res Ther; Cell heterogeneity, rather than the cell storage solution, affects the behavior of mesenchymal stem cells in vitro and in vivo.; Authors - Yong-Hong Wang