Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

Publications

6

CloneNo.

17A2

N° de cat : FITC-65077

Synonymes

AI504783, CD3, Cd3e, CD3epsilon, T3e

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Galerie de données de validation

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  FC experiment of mouse splenocytes using FITC-65077

  1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and 1 ug FITC Plus Anti-Mouse CD3 (FITC-65077, Clone: 17A2) or 1 ug isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 6 publications below

Informations sur le produit

FITC-65077 cible CD3 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Hybridome T-T γδTCR-positif D1
• Nom complet: CD3 antigen, epsilon polypeptide
• Numéro d’acquisition GenBank: BC098236
• Symbole du gène: Cd3e
• Identification du gène (NCBI): 12501
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
mouse	FC	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
mouse	FC	Vet Microbiol; An influenza virus vector candidate vaccine stably expressing SARS-CoV-2 receptor-binding domain produces high and long-lasting neutralizing antibodies in mice.; Authors - Yongzhen Zhao
mouse	FC	Malar J; Analysis of spleen histopathology, splenocyte composition and haematological parameters in four strains of mice infected with Plasmodium berghei K173.; Authors - Huajing Wang
	FC	Liver Int; Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury; Authors - Yuan Li
mouse	FC	Bioact Mater; Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment; Authors - Dongdong Zheng
mouse	FC	Front Pharmacol; In-situ formed thermosensitive hydrogel amplifies statin-mediated immune checkpoint blockade for coordinated tumor chemo-immunotherapy; Authors - Zefan Liu