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Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

OKT3

N° de cat : CL647-65133

Synonymes

CD3, CD3E, T3E, TCRE

Galerie de données de validation

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD3 (OKT3) (CL647-65133)

    FC experiment of human PBMCs using CL647-65133

    1X10^6 human PBMCs were surface co-stained with FITC plus Anti-Human CD19 and 5 ul CoraLite® Plus 647 Anti-Human CD3 (CL647-65133, Clone:OKT3) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD3 (OKT3) (CL647-65133)

    FC experiment of human PBMCs using CL647-65133

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD3 (CL647-65133, Clone:OKT3) (red) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

Applications testées

Résultats positifs en cytométrieCMSP humaines,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65133 cible CD3 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG2a, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Human T cells
Nom complet CD3e molecule, epsilon (CD3-TCR complex)
Masse moléculaire calculée 207 aa, 23 kDa
Numéro d’acquisition GenBankBC049847
Symbole du gène CD3E
Identification du gène (NCBI) 916
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocole

Product Specific Protocols
FC protocol for CL Plus 647 CD3 antibody CL647-65133Download protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sunil (Verified Customer) (07-28-2023)

Worked great as a duplex alongside a blue dye CD4 conjugate we tested with an antibody cocktail on ultracomp beads.

  • Applications: Flow Cytometry
FH

Sareena (Verified Customer) (07-25-2023)

Performed as another commercially available conjugate

  • Applications: Flow Cytometry