Anticorps Monoclonal anti-CD3ε

CD3ε Monoclonal Antibody for FC

Hôte / Isotype

Hamster / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

145-2C11

N° de cat : CL647-65060

Synonymes

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e

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Galerie de données de validation

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  FC experiment of C57BL/6 mouse splenocytes using CL647-65060

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with 0.25 ug FITC Plus Anti-Mouse CD4 (GK1.5) (FITC-65104, Clone: GK1.5) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3ε (CL647-65060, Clone:145-2C11) or 0.5 ug CoraLite® Plus 647 Armenian Hamster IgG Isotype Control (PIP) (CL647-65210, Clone: PIP). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65060 cible CD3ε dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Clone de lymphocyte T cytotoxique (CTL) murin spécifique de H-2Kb, BM10-37
• Nom complet: CD3 antigen, epsilon polypeptide
• Numéro d’acquisition GenBank: BC098236
• Symbole du gène: Cd3e
• Identification du gène (NCBI): 12501
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).