Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

Hit3a

N° de cat : CL555-65112

Synonymes

CD3E, T3E, TCRE

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Galerie de données de validation

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  FC experiment of human PBMCs using CL555-65112

  1X10^6 human PBMCs were surface stained with CL750 Anti-Human CD45 and 5 ul CoraLite® Plus 555 Anti-Human CD3 (CL555-65112, Clone:Hit3a), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL555-65112

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD3 (CL555-65112, Clone:Hit3a), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-65112 cible CD3 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD3e molecule, epsilon (CD3-TCR complex)
• Masse moléculaire calculée: 207 aa, 23 kDa
• Numéro d’acquisition GenBank: BC049847
• Symbole du gène: CD3E
• Identification du gène (NCBI): 916
• Conjugaison: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).