Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications(4)

CloneNo.

17A2

N° de cat : APC-65077

Synonymes

Cd3e, T3e, CD3ε, CD3epsilon, AI504783

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Galerie de données de validation

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  FC experiment of mouse splenocytes using APC-65077

  1x10^6 mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse CD3 (17A2) (APC-65077, Clone:17A2) or 0.1 ug APC Rat IgG2b Isotype Control (LTF-2) (APC-65211, Clone: LTF-2), and 0.1 ug CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 3 publications below

Informations sur le produit

APC-65077 cible CD3 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Hybridome T-T γδTCR-positif D1
• Nom complet: CD3 antigen, epsilon polypeptide
• Numéro d’acquisition GenBank: BC098236
• Symbole du gène: CD3ε
• Identification du gène (NCBI): 12501
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
mouse	FC	J Mater Chem B; PD-1 engineered cytomembrane cloaked molybdenum nitride for synergistic photothermal and enhanced immunotherapy of breast cancer; Authors - Lian Zhu
mouse	FC	Acta Biomater; Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment; Authors - Zhuo Cheng
mouse	FC	J Ethnopharmacol; Ethyl acetate extract from Herpetospermun cardigerum Wall. Ameliorated Concanavalin A-induced autoimmune hepatitis in mice by reprofiling gut microenvironment to modulate IDO1/KYN and PI3K/AKT/NF-κB pathways; Authors - Yu Xiao
		Theranostics; Optimized circular RNA vaccines for superior cancer immunotherapy; Authors - Hongwu Yu

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